PhenoVue Fluor 488 - Concanavalin A is a fluorescent lectin which displays high affinity for glycoproteins and glycolipids present at the cellular membranes. It can be used for cellular membrane staining, particularly the endoplasmic reticulum.
PhenoVue Fluor 488 - Concanavalin A exhibits bright green fluorescence and is validated for use in imaging microscopy and high-content screening applications.
Part of Revvity's portfolio of cellular imaging reagents, PhenoVue Fluor 488 - Concanavalin A has a maximum excitation wavelength of 495 nm and a maximum emission wavelength of 520 nm.
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Concanavalin A is a plant homotetrameric lectin known to activate the immune system or induce apoptosis and autophagy. Concanavalin A displays high affinity for α-mannopyranosyl and α-glucopyranosyl residues of glycoproteins and glycolipids present at the cellular membranes.
Fluorescent Concanavalin A derivatives are commonly used for staining the cellular membranes of mammalian cells, particularly the endoplasmic reticulum.
PhenoVue Fluor 488 - Concanavalin A can be used to visualize cellular membranes in immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.
| Numbers of Vials Per Unit | 5 |
|---|---|
| Quantity or Volume Per Vial | 1mg (29.2nmoles) |
| Form | Lyophilized |
| Storage | 2-8°C |
| Recommended Working Concentration | 1mg (9.62nmoles) |
| Maximum Excitation Wavelength | 495 nm |
| Maximum Emission Wavelength | 520 nm |
| Common filter set | FITC |
| Live Cell Staining | Yes |
| Fixed Cell Staining | Yes. See Product Information Sheet for more information |
| Equivalent Number of Microplates | 1-5 x 96-well microplates 1-5 x 384-well microplates 3-8 x 1536-well microplates |
| Color | Green |
|---|---|
| Detection Method | Fluorescence |
| Filter | FITC |
| Fluorophore | PhenoVue™ Fluor 488 |
| Organelle or Cell Compartment | Plasma membranes, Endoplasmic Reticulum |
| Product Compatibility | Live and fixed samples |
| Product Brand Name | PhenoVue™ |
| Shipping Condition | Ambient |
| Type | Individual Reagent |
| Unit Size | 5 vials |
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This brochure describes Revvity's PhenoVue cellular imaging reagents plus microplates, Imaging instruments, imaging and analysis software and automation.
Fluorescent Concanavalin A conjugates represent a method of choice for labelling the cellular membranes of mammalian cells, particularly the endoplasmic reticulum which is glycoprotein-enriched.
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