Highly cross-adsorbed donkey anti-rabbit IgG (H+L) antibody conjugated with PhenoVue™ Fluor 488.
PhenoVue Fluor 488 secondary antibody conjugates exhibit bright green fluorescence and are validated for use in imaging microscopy and high-content screening applications.
Part of Revvity’s portfolio of cellular imaging reagents, the PhenoVue Fluor - 488 donkey anti-rabbit antibody, highly cross-adsorbed, has a maximum excitation wavelength of 495nm and a maximum emission wavelength of 520nm.
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PhenoVue Fluor 488 - donkey anti-rabbit IgG (H+L) antibody, highly cross-adsorbed, has been cross-adsorbed against various IgG species such as rat, mouse, hamster, human, bovine, horse, or chicken serum to minimize cross-reactivity with other species. Specificity and sensitivity are improved and background reduced with PhenoVue Fluor 488 - donkey anti-rabbit IgG (H+L) antibody, highly cross-adsorbed.
It can be used in indirect immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.
| Numbers of Vials Per Unit | 1 |
|---|---|
| Quantity or Volume Per Unit | 1 mg |
| Form | Lyophilized |
| Storage | 2-8°C |
| Recommended Working Concentration | 1.5 µg/mL (10nM) |
| Maximum Excitation Wavelength | 495 nm |
| Maximum Emission Wavelength | 520 nm |
| Common Filter Set | FITC |
| Live Cell Staining | No |
| Fixed Cell Staining | Yes |
| Equivalent Number of Microplates |
25-70 x 96-well microplates |
| Color | Green |
|---|---|
| Detection Method | Fluorescence |
| Filter | FITC |
| Fluorophore | PhenoVue™ Fluor 488 |
| Host | Donkey |
| Product Compatibility | Fixed samples only |
| Product Brand Name | PhenoVue™ |
| Shipping Condition | Ambient |
| Target Species | Rabbit |
| Type | Individual Reagent |
| Unit Size | 1 vial |
This brochure describes Revvity's PhenoVue cellular imaging reagents plus microplates, Imaging instruments, imaging and analysis software and automation.
Donkey anti-rabbit IgG (H+L) antibodies are conjugated with our bright PhenoVue Fluor dyes.
PhenoVue Fluor 488 - Donkey Anti-Rabbit Antibody Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using PhenoVue Fluor 488 - Donkey Anti-Rabbit IgG Antibody Highly Cross-Adsorbed
A431 cells stained with PhenoVue Fluor 488 - Donkey Anti-Rabbit Antibody Highly Cross-Adsorbed
PhenoVue Fluor 488 - Donkey Anti-Rabbit Antibody Highly Cross-Adsorbed
