PhenoVue Fluor 488 - Phalloidin is a fluorescent dye conjugated bicyclic peptide which binds specifically to F-actin and stains the actin cytoskeleton.
PhenoVue Fluor 488 conjugated Phalloidin exhibits bright green fluorescence and is validated for use in imaging microscopy and high-content screening applications.
Part of Revvity's portfolio of cellular imaging reagents, PhenoVue 488 - Phalloidin has a maximum excitation wavelength of 495 nm and a maximum emission wavelength of 520 nm.
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Phalloidin is a toxin isolated from the fungus Amanita phalloides. It is a bicyclic peptide that specifically binds and stabilizes actin filaments.
Fluorescent Phalloidin derivatives have been extensively used for monitoring actin expression and dynamics of the cytoskeleton network. The study of actin cytoskeleton regulation is of interest in the context of several pathophysiological processes such as cancer and neurodegenerative diseases as well as fibrosis and vascular diseases.
PhenoVue Fluor 488 - Phalloidin can be used to vizualise actin filaments in indirect immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.
| Numbers of Vials Per Unit | 1 |
|---|---|
| Quantity or Volume Per Vial | 300 units (10nmoles; 13µg) |
| Form | Dessicated |
| Storage | -20°C |
| Recommended Working Concentration | 165nM (0.21µg/mL) |
| Maximum Excitation Wavelength | 495 nm |
| Maximum Emission Wavelength | 520 nm |
| Common Filter Set | FITC |
| Live Cell Staining | No |
| Fixed Cell Staining | Yes |
| Equivalent Number of Microplates | 2-6 x 96-well microplates 1.5-6 x 384-well microplates 3-10 x 1536-well microplates |
| Color | Green |
|---|---|
| Detection Method | Fluorescence |
| Filter | FITC |
| Fluorophore | PhenoVue™ Fluor 488 |
| Organelle or Cell Compartment | Actin |
| Product Compatibility | Fixed samples only |
| Product Brand Name | PhenoVue™ |
| Shipping Condition | Dry Ice |
| Type | Individual Reagent |
| Unit Size | 1 vial |
Cell Painting is a powerful high-content screening method which combines cell and computational biology to unravel cells’ responses and gain a deeper understanding of the effects of chemical and genetic perturbagens.
However, implementation of cell painting is not without its challenges - f ...
This brochure describes Revvity's PhenoVue cellular imaging reagents plus microplates, Imaging instruments, imaging and analysis software and automation.
This is a product information sheet for PhenoVue Fluor - Phalloidin Conjugates
PhenoVue Fluor 488 - Phalloidin
PhenoVue Fluor 488 - Phalloidin
HeLa cells stained with PhenoVue Fluor 488 - Phalloidin
HeLa cells stained with PhenoVue Fluor 488 - Phalloidin
HeLa fixed and permeabilized cells stained with PhenoVue Fluor 488 Phalloidin (actin, green). Imaged on Operetta CLS.
HeLa fixed and permeabilized cells stained with PhenoVue Fluor 488 Phalloidin (actin, green) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
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