PhenoVue Fluor 555 - Concanavalin A is a fluorescent lectin which displays high affinity for glycoproteins and glycolipids present at the cellular membranes. It can be used for cellular membrane staining, particularly the endoplasmic reticulum. PhenoVue Fluor 555 - Concanavalin A exhibits bright green fluorescence and is validated for use in imaging microscopy and high-content screening applications. Part of Revvity's portfolio of cellular imaging reagents, PhenoVue Fluor 555 - Concanavalin A has a maximum excitation wavelength of 555 nm and a maximum emission wavelength of 570 nm.
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Concanavalin A is a plant homotetrameric lectin known to activate the immune system or induce apoptosis and autophagy. Concanavalin A displays high affinity for α-mannopyranosyl and α-glucopyranosyl residues of glycoproteins and glycolipids present at the cellular membranes. Fluorescent Concanavalin A derivatives are commonly used for staining the cellular membranes of mammalian cells, particularly the endoplasmic reticulum. PhenoVue Fluor 555 - Concanavalin A can be used to visualize cellular membranes in immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.
| Numbers of Vials Per Unit | 5 |
|---|---|
| Quantity or Volume Per Unit | 1mg (9.62nmoles) |
| Form | Lyophilized |
| Storage | 2 - 8°C |
| Recommended Working Concentration | 100 µg/mL |
| Maximum Excitation Wavelength | 555 nm |
| Maximum Emission Wavelength | 570 nm |
| Common Filter Set | Cy3 |
| Live Cell Staining | Yes |
| Fixed Cell Staining | Yes. See Product Information Sheet for more information |
| Equivalent Number of Microplates | 1 - 5 x 96-well microplates 1 - 5 x 384-well microplates 3 - 8 x 1536-well microplates |
| Color | Yellow/Orange |
|---|---|
| Detection Method | Fluorescence |
| Filter | Cy3 |
| Fluorophore | PhenoVue™ Fluor 555 |
| Organelle or Cell Compartment | Plasma membranes, Endoplasmic Reticulum |
| Product Compatibility | Live and fixed samples |
| Product Brand Name | PhenoVue™ |
| Shipping Condition | Ambient |
| Type | Individual Reagent |
| Unit Size | 5 vials |
This brochure describes Revvity's PhenoVue cellular imaging reagents plus microplates, Imaging instruments, imaging and analysis software and automation.
Fluorescent Concanavalin A conjugates represent a method of choice for labelling the cellular membranes of mammalian cells, particularly the endoplasmic reticulum which is glycoprotein-enriched.
PhenoVue Fluor 647 - Concanavalin A
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PhenoVue Fluor 488 - Concanavalin A
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