PhenoVue™ Fluor 555 - Phalloidin is a fluorescent dye conjugated bicyclic peptide which binds specifically to F-actin and stains the actin cytoskeleton.
PhenoVue Fluor 555 conjugated Phalloidin exhibits bright green fluorescence and is validated for use in imaging microscopy and high-content screening applications.
Part of 爱游戏平台注册登录 's portfolio of cellular imaging reagents, PhenoVue 555 - Phalloidin has a maximum excitation wavelength of 555 nm and a maximum emission wavelength of 570 nm.
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Phalloidin is a toxin isolated from the fungus Amanita phalloides. It is a bicyclic peptide that specifically binds and stabilizes actin filaments.
Fluorescent Phalloidin derivatives have been extensively used for monitoring actin expression and dynamics of the cytoskeleton network. The study of actin cytoskeleton regulation is of interest in the context of several pathophysiological processes such as cancer and neurodegenerative diseases as well as fibrosis and vascular diseases.
PhenoVue Fluor 555 - Phalloidin can be used to vizualise actin filaments in indirect immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.
| Numbers of Vials Per Unit | 1 |
|---|---|
| Quantity or Volume Per Unit | 300 units (10nmoles; 13µg) |
| Form | Dessicated |
| Storage | -20°C |
| Recommended Working Concentration | 165nM (0.21µg/mL) |
| Maximum Excitation Wavelength | 555nm |
| Maximum Emission Wavelength | 570nm |
| Common Filter Set | Cy3 |
| Live Cell Staining | No |
| Fixed Cell Staining | Yes |
| Equivalent Number of Microplates | 2 - 6 x 96-well microplates 1.5 - 6 x 384-well microplates 3 - 10 x 1536-well microplates |
| Color | Red |
|---|---|
| Detection Method | Fluorescence |
| Filter | Texas-Red |
| Fluorophore | PhenoVue™ Fluor 555 |
| Organelle or Cell Compartment | Actin |
| Product Compatibility | Fixed samples only |
| Product Brand Name | PhenoVue™ |
| Shipping Condition | Dry Ice |
| Type | Individual Reagent |
| Unit Size | 1 vial |
This brochure describes 爱游戏平台注册登录 's PhenoVue cellular imaging reagents plus microplates, Imaging instruments, imaging and analysis software and automation.
This is a product information sheet for PhenoVue Fluor - Phalloidin Conjugates
PhenoVue Fluor 555 - Phalloidin
PhenoVue Fluor 555 - Phalloidin
HeLa cells stained with PhenoVue Fluor 555 - Phalloidin
HeLa (Human Epithelial) fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with 55 nM PhenoVue Fluor 555 Phalloidin (actin, orange). Imaged on Operetta CLS, 63X Water Confocal.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 555 Phalloidin (actin, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
