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PhenoVue™ Fluor 555 - WGA is a fluorescent lectin which displays high affinity for sialic acid and N-acetylglucosamine residues of glycoproteins and glycolipids present at the cellular plasma membranes. It can be used for cellular membrane staining, particularly the Golgi apparatus.
PhenoVue Fluor 555 - WGA exhibits bright orange fluorescence and is validated for use in imaging microscopy and high-content screening applications.
Part of 爱游戏平台注册登录 's portfolio of cellular imaging reagents, PhenoVue Fluor 555 - WGA has a maximum excitation wavelength of 555 nm and a maximum emission wavelength of 570 nm.
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WGA is a plant lectin that binds different carbohydrate motifs and displays high affinity for sialic acid and N-acetylglucosamine residues of glycoproteins and glycolipids present at the cellular plasma membranes.
Fluorescent WGA derivatives are commonly used for staining the cellular membranes of mammalian cells, particularly Golgi apparatus which is glycoprotein-enriched. In addition, fluorescent WGA derivatives are also routinely used for the staining of skeletal muscle cells and tissues, as well as in cardiac fibrosis.
PhenoVue Fluor 555 - WGA can be used to visualize cellular membranes in immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.
| Numbers of Vials Per Unit | 5 |
|---|---|
| Quantity or Volume Per Unit | 1mg (29.2nmoles) |
| Form | Lyophilized |
| Storage | 2 - 8°C |
| Recommended Working Concentration | 5 µg/mL (146nM ) |
| Maximum Excitation Wavelength | 555nm |
| Maximum Emission Wavelength | 570nm |
| Common Filter Set | Cy3 |
| Live Cell Staining | Yes |
| Fixed Cell Staining | Yes. See Product Information Sheet for more information |
| Equivalent Number of Microplates | 36 - 100 x 96-well microplates 30 - 100 x 384-well microplates 55 - 160 x 1536-well microplates |
| Color | Yellow/Orange |
|---|---|
| Detection Method | Fluorescence |
| Filter | Cy3 |
| Fluorophore | PhenoVue™ Fluor 555 |
| Organelle or Cell Compartment | Golgi Apparatus |
| Product Compatibility | Live and fixed samples |
| Product Brand Name | PhenoVue™ |
| Shipping Condition | Ambient |
| Type | Individual Reagent |
| Unit Size | 5 vials |
This brochure describes 爱游戏平台注册登录 's PhenoVue cellular imaging reagents plus microplates, Imaging instruments, imaging and analysis software and automation.
Fluorescent WGA conjugates represent a method of choice for labelling the cellular membranes of mammalian cells, particularly Golgi apparatus which is glycoprotein enriched.
PhenoVue Fluor 555 - WGA
PhenoVue Fluor 555 - WGA
HeLa cells stained with PhenoVue Fluor 555 - WGA
HeLa cells stained with PhenoVue Fluor 555 - WGA
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 555 - WGA (Plasma Membrane + Golgi Appartus, orange) (in PhenoVue Dye Diluent A). Imaged on Operetta CLS.
HeLa fixed cells stained with PhenoVue Fluor 555 WGA (Plasma Membrane, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
HeLa fixed cells stained with PhenoVue Fluor 555 WGA (Plasma Membrane, orange). Imaged on Operetta CLS.
