Highly cross-adsorbed goat anti-mouse IgG (H+L) antibody conjugated with PhenoVue™ Fluor 568.
PhenoVue fluor 568 secondary antibody conjugates exhibit bright red-orange fluorescence and are validated for use in high-content screening applications.
Part of Revvity’s portfolio of cellular imaging reagents, the PhenoVue Fluor - 568 goat anti-mouse antibody, highly cross-adsorbed, has a maximum excitation wavelength of 578nm and a maximum emission wavelength of 603nm.
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PhenoVue Fluor 568 - goat anti-mouse IgG (H+L) antibody, highly cross-adsorbed, has been cross-adsorbed against various IgG species such as rat, hamster, human, rabbit bovine, horse, or chicken serum to minimize cross-reactivity with other species.
Specificity and sensitivity are improved and background reduced with PhenoVue Fluor 568 - goat anti-mouse IgG (H+L) antibody, highly cross-adsorbed.
It can be used in indirect immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.
| Numbers of Vials Per Unit | 1 |
|---|---|
| Quantity or Volume Per Unit | 1 mg |
| Form | Lyophilized |
| Storage | 2-8°C |
| Recommended Working Concentration | 1.5 µg/mL (10nM) |
| Maximum Excitation Wavelength | 578 nm |
| Maximum Emission Wavelength | 603 nm |
| Common Filter Set | Texas-Red |
| Live Cell Staining | No |
| Fixed Cell Staining | Yes |
| Equivalent Number of Microplates | 25-70 x 96-well microplates 20-70 x 384-well microplates 35-90 x 1536-well microplates |
| Color | Red |
|---|---|
| Detection Method | Fluorescence |
| Filter | Texas-Red |
| Fluorophore | PhenoVue™ Fluor 568 |
| Host | Goat |
| Product Compatibility | Fixed samples only |
| Product Brand Name | PhenoVue™ |
| Shipping Condition | Ambient |
| Target Species | Mouse |
| Type | Individual Reagent |
| Unit Size | 1 vial |
This brochure describes Revvity's PhenoVue cellular imaging reagents plus microplates, Imaging instruments, imaging and analysis software and automation.
This is a product information sheet for PhenoVue Fluor - Goat anti-Mouse Secondary Antibodies.
PhenoVue Fluor 568 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed
PhenoVue Fluor 568 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using PhenoVue Fluor 568 Goat Anti-Mouse IgG Cross-Adsorbed and Highly Cross-Adsorbed
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 568 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, orange). Imaged on Operetta CLS.
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 568 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, orange) + PhenoVue Hoechst 33342 (nucleus, blue). Imaged on Operetta CLS.
Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 568 Goat Anti-Mouse IgG Highly Cross-Adsorbed
PhenoVue Hoechst 33342 Nuclear Stain 100mg
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PhenoVue 551 Mitochondrial Stain
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PhenoVue Fluor 594 - Phalloidin
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PhenoVue Fluor 568 - Goat Anti-Mouse Antibody Cross-Adsorbed
List Price : USD196.0
