PhenoVue Fluor 568 - Phalloidin is a fluorescent dye conjugated bicyclic peptide which binds specifically to F-actin and stains the actin cytoskeleton.
PhenoVue Fluor 568 conjugated Phalloidin exhibits bright red orange fluorescence and is validated for use in imaging microscopy and high-content screening applications.
Part of 爱游戏平台注册登录 's portfolio of cellular imaging reagents, PhenoVue 568 - Phalloidin has a maximum excitation wavelength of 578 nm and a maximum emission wavelength of 603 nm.
false falseFor research use only
Phalloidin is a toxin isolated from the fungus Amanita phalloides. It is a bicyclic peptide that specifically binds and stabilizes actin filaments.
Fluorescent Phalloidin derivatives have been extensively used for monitoring actin expression and dynamics of the cytoskeleton network. The study of actin cytoskeleton regulation is of interest in the context of several pathophysiological processes such as cancer and neurodegenerative diseases as well as fibrosis and vascular diseases.
PhenoVue Fluor 568 - Phalloidin can be used to vizualise actin filaments in indirect immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.
| Numbers of Vials Per Unit | 1 |
|---|---|
| Quantity or Volume Per Vial | 300 units (10nmoles; 15µg) |
| Form | Dessicated |
| Storage | -20°C |
| Recommended Working Concentration | 165nM (0.25µg/mL) |
| Maximum Excitation Wavelength | 578 nm |
| Maximum Emission Wavelength | 603 nm |
| Common Filter Set | FITC |
| Live Cell Staining | No |
| Fixed Cell Staining | Yes |
| Equivalent Number of Microplates | 2-6 x 96-well microplates 1.5-6 x 384-well microplates 3-10 x 1536-well microplates |
| 顏色 | Yellow/Orange |
|---|---|
| 檢測方法 | Fluorescence |
| Aequorian親本細胞株 | Cy3 |
| 熒光基團 | PhenoVue™ Fluor 568 |
| CELL | Actin |
| 型號兼容 | Fixed samples only |
| 產品品牌名稱 | PhenoVue™ |
| 運輸條件 | 幹冰 |
| Type | Individual Reagent |
| 產品尺寸 | 1 vial |
This is a product information sheet for PhenoVue Fluor - Phalloidin Conjugates
This brochure describes Revvity's PhenoVue cellular imaging reagents plus microplates, Imaging instruments, imaging and analysis software and automation.
Cell Painting is a powerful high-content screening method which combines cell and computational biology to unravel cells’ responses and gain a deeper understanding of the effects of chemical and genetic perturbagens.
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PhenoVue Fluor 568 - Phalloidin
PhenoVue Fluor 568 - Phalloidin
HeLa cells stained with PhenoVue Fluor 568 - Phalloidin
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 568 Phalloidin (actin, orange). Imaged on Operetta CLS.
HeLa fixed (PFA) and permeabilized (0.1% Triton X100) cells stained with PhenoVue Fluor 568 Phalloidin (actin, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
