Highly cross-adsorbed goat anti-mouse IgG (H+L) antibody conjugated with PhenoVue™ Fluor 647.
PhenoVue Fluor 647 secondary antibody conjugates exhibit bright red-orange fluorescence and are validated for use in high-content screening applications.
Part of 爱游戏平台注册登录 ’s portfolio of cellular imaging reagents, the PhenoVue Fluor - 647 goat anti-mouse antibody, highly cross-adsorbed, has a maximum excitation wavelength of 650nm and a maximum emission wavelength of 670nm.
false falsePhenoVue Fluor 647 - goat anti-mouse IgG (H+L) antibody, highly cross-adsorbed, has been cross-adsorbed against various IgG species such as rat, hamster, human, rabbit bovine, horse, or chicken serum to minimize cross-reactivity with other species.
Specificity and sensitivity are improved and background reduced with PhenoVue Fluor 647 - goat anti-mouse IgG (H+L) antibody, highly cross-adsorbed.
It can be used in indirect immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.
| Numbers of Vials Per Unit | 1 |
|---|---|
| Quantity or Volume Per Unit | 1 mg |
| Form | Lyophilized |
| Storage | 2-8°C |
| Recommended Working Concentration | 1.5 µg/mL(10nM) |
| Maximum Excitation Wavelength | 650nm |
| Maximum Emission Wavelength | 670nm |
| Common Filter Set | Cy5 |
| Live Cell Staining | No |
| Fixed Cell Staining | Yes |
| Equivalent Number of Microplates | 25-70 x 96-well microplates 20-70 x 384-well microplates 35-90 x 1536-well microplates |
| 顏色 | Red |
|---|---|
| 檢測方法 | Fluorescence |
| Aequorian親本細胞株 | Cy5 |
| 熒光基團 | PhenoVue™ Fluor 647 |
| 宿主細胞 | Goat |
| 型號兼容 | Fixed samples only |
| 產品品牌名稱 | PhenoVue™ |
| 運輸條件 | 環境 |
| Target Species | Mouse |
| Type | Individual Reagent |
| 產品尺寸 | 1 vial |
This is a product information sheet for PhenoVue Fluor - Goat anti-Mouse Secondary Antibodies.
This brochure describes 爱游戏平台注册登录 's PhenoVue cellular imaging reagents plus microplates, Imaging instruments, imaging and analysis software and automation.
PhenoVue Fluor 647 - Goat Anti-Mouse Antibody, Highly Cross-Adsorbed
PhenoVue Fluor 647 - Goat Anti-Mouse Antibody, Highly Cross-Adsorbed
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Detection of EGFR expressed in A431 cells by indirect immunofluorescence using PhenoVue Fluor 647 - Goat Anti-Mouse IgG Cross-Adsorbed and Highly Cross- Adsorbed
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 647 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, orange). Imaged on Operetta CLS.
A431 cells fixed and permeabilized, stained with Mouse anti EGFR antibody and PhenoVue Fluor 647 - Goat Anti-Mouse Antibody Highly Cross-Adsorbed (EGFR receptor, orange) + PhenoVue Hoechst 33342 (nucleus, blue). Imaged on Operetta CLS.
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Detection of EGFR expressed in A431 cells by indirect immunofluorescence using increasing concentrations of PhenoVue Fluor 647 - Goat Anti-Mouse IgG Highly Cross-Adsorbed
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