PhenoVue Nile Red Lipid stain is a lipophilic organic molecule commonly used for localization and quantification of intracellular lipid droplets.
PhenoVue Nile Red Lipid stain exhibits bright yellow fluorescence and is validated for use in imaging microscopy and high-content screening applications.
PhenoVue Nile Red Lipid stain exhibits:
For research use only
PhenoVue Nile Red Lipid stain is a lipophilic organic molecule that is almost nonfluorescent in water and polar solvents. In lipid-rich environments, Nile Red exhibits enhanced yellow fluorescence, as well as red fluorescence to a lesser extent.
Nile Red is commonly used for localization and quantification of intracellular lipid droplets which are involved in lipid synthesis, metabolism and transportation as well as protein storage and degradation or viral replication, all of which are related to pathophysiology, including dyslipidemia, obesity, lipodystrophy, diabetes, fatty liver diseases, or atherosclerosis.
| Numbers of Vials Per Unit | 2 |
|---|---|
| Quantity or Volume Per Vial | 10mg (31.4µmoles) |
| Form | Powder |
| Storage | -20°C |
| Recommended Working Concentration | 200nM (0.008ng/mL) |
| Maximum Excitation Wavelength |
512 nm - complexed with triglycerides |
| Maximum Emission Wavelength | 585 nm - complexed with triglycerides 638 nm - complexed with phospholipids* (*as shown in SpectraViewer below) |
| Common Filter Set | Cy3.5 |
| Live Cell Staining | Yes |
| Fixed Cell Staining | Yes. See Product Information Sheet for more information |
| Equivalent Number of Microplates | 10900-32700 x 96-well microplates 9090-32700 x 384-well microplates 17040-40900 x 1536-well microplates |
| 顏色 | Yellow/Orange |
|---|---|
| 檢測方法 | Fluorescence |
| Aequorian親本細胞株 | Cy3 |
| CELL | Lipid droplets |
| 型號兼容 | Live and fixed samples |
| 產品品牌名稱 | PhenoVue™ |
| 運輸條件 | 環境 |
| Type | Individual Reagent |
| 產品尺寸 | 2 vials |
This is a product information sheet for PhenoVue Nile Red Lipid Stain.
This brochure describes 爱游戏平台注册登录 's PhenoVue cellular imaging reagents plus microplates, Imaging instruments, imaging and analysis software and automation.
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PhenoVue Nile Red Lipid Stain
PhenoVue Nile Red Lipid Stain
HeLa cells stained with PhenoVue Nile Red
HepG2 cells treated with Oleic Acid-BSA then fixed (PFA) and stained with PhenoVue Nile-Red Lipid Stain (Lipid Droplets, orange). Imaged on Operetta CLS.
HepG2 cells treated with Oleic Acid-BSA then fixed (PFA) and stained with PhenoVue Nile-Red Lipid Stain (Lipid Droplets, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Operetta CLS.
