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Alpha SureFire® Ultra™ Multiplex p-EGFR (Tyr1068) (Eu) + Total EGFR (Tb) Kit, 10,000 Assay Points

The Alpha SureFire® Ultra™ Multiplex p-EGFR (Tyr1068) + Total EGFR assay kit is used to measure both phosphorylated and total levels of EGFR in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure EGFR (Tyr1068) activation by either recombinant or endogenous receptors, and can be applied to primary cells.

For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

貨號
產品尺寸
MPSU-PTEGFR-K-HV
100 Assay Points
更多
MPSU-PTEGFR-K500
500 Assay Points
更多
MPSU-PTEGFR-K10K
10,000 Assay Points
更多
MPSU-PTEGFR-K50K
50,000 Assay Points
更多
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詳細信息

The Alpha SureFire Ultra Multiplex p-EGFR + Total EGFR assay kit is used to measure both phosphorylated and total levels of endogenous EGFR in cellular lysates in a multiplexing Alpha no-wash assay. No specially engineered cell lines are required. The 615 nm (Eu) signal corresponds to the phosphorylated EGFR analysis, and the 545 nm (Tb) signal corresponds to the total EGFR analysis. This kit has been formulated to provide superior signal:background assay windows and to perform without interference in the presence of extraneous antibodies, making it amenable to the study of therapeutic and blocking antibodies.

  • No-wash steps, no separation steps
  • Multiplexing assay
  • Sensitive detection
  • No interference from exogenous antibodies
  • Results in less than 3 hours

In this assay, the Alpha 615 Acceptor bead is coated with the CaptSure™ antibody, which binds the CaptSure-tagged anti-phospho target antibody. The Alpha 545 Acceptor bead is coated with the CaptSure2™ agent, which binds the CaptSure2 tagged anti-total target protein antibody. The Alpha Donor bead binds the biotinylated anti-total target protein antibody.

規格

檢測目標 EGFR
檢測目標類 Phosphoprotein, Protein
自動化兼容 Yes
檢測方法 Alpha
產品品牌名稱 AlphaLISA SureFire Ultra Multiplex
運輸條件 藍冰
產品尺寸 10,000 Assay Points
資源
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產品手冊

應用文獻

Alpha Terbium SureFire UltraMultiplex: Simultaneous Dual Phosphoprotein Target Analysis

Evaluating pharmaceutical compound efficacy and safety in regulating cell behavior can involve the study of multiple signal transduction pathways and measuring more than one cellular target can provide greater confidence of positive compound hits on the cellular target of interest. Protein phosph ...

PDF 1 MB
Simultaneous Detection of Total and Phosphorylated Protein in Cellular Kinase Assay Using Alpha SureFire Ultra Multiplex Technology

Cellular kinase signal transduction pathways are involved in the regulation of many important cellular processes such as cell survival, differentiation, and apoptosis. Kinase signaling networks are typically characterized by multiple kinases arranged in cascades containing nodes with feedback loo ...

PDF 1 MB
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