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Thymidine, [Methyl-3H]-, Specific Activity: 2 Ci/mmol, 250 µCi

Thymidine, tritiated on the methyl group. This product is supplied steri-packaged in an aqueous solution, and is typically used in DNA cell proliferation assays.

Part Number
Unit Size
List Price
Your Price
Quantity
NET027A250UC
250 µCi
662.00 USD
 
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NET027A001MC
1 mCi
1187.00 USD
 
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NET027A005MC
5 mCi
2861.00 USD
 
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Overview

250 µCi quantities of [Methyl-3H]-Thymidine are available for your research. Application of [3H]Thymidine can be found in: carboxy-terminal PTH fragments stimulating incorporation in vascular endothelial cells, quantification of granulocyte-macrophage colony-stimulating factor (GM-CSF) hypersensitivity in juvenile myelomonocytic leukemia (JMML), influence of parasympathetic non-adrenergic, non-cholinergic mechanisms on uptake (rat) in response to mastication, measurement of dendritic cell induced T cell response by intracellular ATP levels and uptake in human immunology, etc.

Special Information:
If aqueous solutions of thymidine are stored frozen (-20°C), care should be taken to protect the material from U.V. light during the thawing process to prevent possible dimerization.

When [methyl-3H]thymidine is incorporated into cellular DNA and undergoes radiolytic decomposition, [3H]5-hydroxymethyl-2''-deoxyuridine (HMdU) is formed.* The formation of HMdU through transmutation of [methyl-3H]thymidine occurs at the rate of B-decay, which is 0.017% per day. The transmutation product is not volatile.

*References
S. Apelgot and B. Ekert, J. Chem. Phys. 60, 505 (1963).
K. Fink, R. E. Cline, and R. M. Fink, Anal. Chem., 35, 389 (1963).
Specifications and Criteria for Biochemical Compounds, Third Edition (ISBN 0-309-01917-6), National Academy of Sciences - National Research Council, Washington, D.C. 1972.
G. W. Teebor, K. Frenkel, and M. S. Goldstein, Proc. Natl. Acad. Sci. USA 81, 318 (1984).
H. Tono, S.S. Cohen, J. Biol. Chem. 237, 1271 (1962).
M. Nemer, J. Biol. Chem. 237, 143 (1962).

Specifications

Concentration 1 mCi/mL
Detection Method Radiometric
Label Position Specifically Labeled
Molecular Weight 242.2
Product Brand Name NEN Radiochemicals
Radioisotope H-3
Radionuclide 3H
Shipping Condition Ambient
Special Ordering Information This is a radioactive product - shipping address must have a license to receive radioactive materials.
Unit Size 250 µCi
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Radiometric Reagents Guide 2010-2011

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