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Thymidine, [Methyl-3H]-, Specific Activity: 70-90Ci (2.59-3.33TBq)/mMole,>97%, 1mCi (37MBq)

Thymidine, tritiated on the methyl group. This product is supplied steri-packaged in an aqueous solution, and is typically used in DNA cell proliferation assays.

貨號
產品尺寸
NET027Z250UC
250 µCi
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NET027Z001MC
1 mCi
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NET027Z005MC
5 mCi
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詳細信息

250 µCi quantities of [Methyl-3H]-Thymidine are available for your research. Application of [3H]Thymidine can be found in: carboxy-terminal PTH fragments stimulating incorporation in vascular endothelial cells, quantification of granulocyte-macrophage colony-stimulating factor (GM-CSF) hypersensitivity in juvenile myelomonocytic leukemia (JMML), influence of parasympathetic non-adrenergic, non-cholinergic mechanisms on uptake (rat) in response to mastication, measurement of dendritic cell induced T cell response by intracellular ATP levels and uptake in human immunology, etc.

Special Information:
If aqueous solutions of thymidine are stored frozen (-20°C), care should be taken to protect the material from U.V. light during the thawing process to prevent possible dimerization.

When [methyl-3H]thymidine is incorporated into cellular DNA and undergoes radiolytic decomposition, [3H]5-hydroxymethyl-2''-deoxyuridine (HMdU) is formed.* The formation of HMdU through transmutation of [methyl-3H]thymidine occurs at the rate of B-decay, which is 0.017% per day. The transmutation product is not volatile.

*References
S. Apelgot and B. Ekert, J. Chem. Phys. 60, 505 (1963).
K. Fink, R. E. Cline, and R. M. Fink, Anal. Chem., 35, 389 (1963).
Specifications and Criteria for Biochemical Compounds, Third Edition (ISBN 0-309-01917-6), National Academy of Sciences - National Research Council, Washington, D.C. 1972.
G. W. Teebor, K. Frenkel, and M. S. Goldstein, Proc. Natl. Acad. Sci. USA 81, 318 (1984).
H. Tono, S.S. Cohen, J. Biol. Chem. 237, 1271 (1962).
M. Nemer, J. Biol. Chem. 237, 143 (1962).

規格

檢測方法 Radiometric
標簽位置 Specifically Labeled
分子量 242.2
產品品牌名稱 NEN Radiochemicals
放射性同位素 H-3
放射性核素 3H
運輸條件 環境
特殊訂購信息 This is a radioactive product - shipping address must have a license to receive radioactive materials.
產品尺寸 1 mCi
資源
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產品手冊

Radiometric Reagents Guide 2010-2011

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