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LANCE Ultra ULight™-Acetyl-CoA Carboxylase (Ser79) Peptide, 5 nmole

Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight-labeled synthetic peptide derived from residues 73-85 of rat Acetyl-CoA Carboxylase in which Ser77 is mutated to Ala (also known as SAMS). The ULight-Acetyl-CoA Carboxylase peptide can be phosphorylated at Ser 79 by the AMPKα1 kinase. Peptide sequence : CHMRSAMSGLHLVKRR. Please note this product replaces TRF0118-D or TRF0118-M, now being supplied lyophilized rather than in liquid (frozen) format.

For research use only. Not for use in diagnostic procedures.

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TRF0133-D
0.5 nmol
569.00 USD
 
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TRF0133-M
5 nmol
1629.00 USD
 
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Overview

LANCE Ultra TR-FRET technology provides a two-component kinase assay that combines direct labeling of the kinase substrate with ULight™, an innovative emission dye for optimum binding efficiency, reducing steps and assay prep time. Validated for over 310 kinases, we provide data transparency by supplying:

  • Test conditions: Rapid assay setup to move straight into optimized assay performance
  • S:B data: Confidence in proven performance of the kinase on specific substrates; no guessing
  • Phospho-motifs: Substrate sequences are provided
  • Assay options: Multiple substrates are tested so you can select the best peptide sequence and S:B for your kinase

For ULight substrates:

  • 0.5 nmoles = 1,000 assay points
  • 5 nmoles = 10,000 assay points

In LANCE Ultra kinase assays, the binding of a Eu-labeled anti-phosphosubstrate antibody to the phosphorylated ULight-labeled substrate brings donor and acceptor molecules into close proximity. After irradiation of the kinase reaction at 320 or 340 nm, the energy from the Eu donor is transferred to the ULight acceptor dye, which in turn generates light at 665 nm. The intensity of the light emission is proportional to the level of ULight-substrate phosphorylation.

Specifications

Assay Target Class Phosphoprotein
Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), TR-FRET
Product Brand Name LANCE Ultra
Shipping Condition Dry Ice
Therapeutic Area Cardiovascular
Unit Size 5 nmol
Resources, Events & More
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Application Note

Comparison of LANCE Ultra TR-FRET to 爱游戏平台注册登录 's Classical LANCE TR-FRET Platform for Kinase Applications

Protein kinases play a major role in normal cellular functions such as cell proliferation, angiogenesis, and cell adhesion. Dysregulation of kinase activity has been shown to be associated with several human diseases including cancer, diabetes and morphological disorders. This makes kinases crucial ...

PDF 805 KB
Development and Optimization of Kinase Assays using New LANCE Ultra TR-FRET Reagents

Dysregulation of kinase activity has been shown to be associated with several human diseases including cancer, diabetes and morphological disorders. Because of their function, kinases are crucial targets for drug discovery. Analysis of the human genomehas revealed the existence ofnearly 520 genes en ...

PDF 583 KB
Improved Stability of the LANCE Ultra Signal in Kinase Assays

LANCE® Ultra is a high through putscreening (HTS) technology platform optimized for homogeneous time-resolved fluorescence resonance energy transfer (TRFRET) kinase assays. The LANCEUltra donor dye is a Europium(Eu) chelate, and the acceptor dye is a proprietary small-molecular weight molecule calle ...

PDF 206 KB

Brochure

Guide

LANCE Ultra Substrate Selection Guide

LANCE® Ultra TR-FRET reagents comprise the widest portfolio of validated kinase assay offerings available for rapid, sensitive and robust screening of purified kinase targets in a biochemical format.

PDF 1 MB
LANCE® Ultra Kinase Assay Selection Guide

LANCE® Ultra TR-FRET reagents comprise the widest portfolio of validated kinase assay offerings available for rapid, sensitive ,and robust screening of purified kinase targets in a biochemical format.

PDF 1 MB

Poster

Comparison of LANCE Ultra with Other TR-FRET Platforms

Protein kinases play a major role in normal cellular functions such as cell proliferation, angiogenesis, and cell adhesion. Dysregulation of kinase activity has been shown to be associated with several human diseases including cancer, diabetes and morphological disorders. This makes kinases crucial ...

PDF 356 KB
Development, Automation and Miniaturization of High-Throughput Serine/Threonine Kinase Assays Using the LANCE® Ultra Platform

Protein kinases are involved in the regulation of many aspects of the cellcycle, including proliferation, differentiation, secretion and apoptosis. Aberrant protein kinase expression or functioning is a cause orconsequence of many human diseases. As a result, kinases have become extremely popular fo ...

PDF 364 KB
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