Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. MBP peptide (sequence: CFFKNIVTPRTPPPSQGK-amide) labeled with the ULight™ dye. Human Myelin Basic Protein (MBP) phosphorylation site: Thr232 (Swiss-Prot: P02686); corresponds to Thr98 in other isoforms or species. MBP can be phosphorylated by a variety of kinases including ERK1/ERK2.
false falseFor research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
LANCE Ultra TR-FRET technology provides a two-component kinase assay that combines direct labeling of the kinase substrate with ULight™, an innovative emission dye for optimum binding efficiency, reducing steps and assay prep time. Validated for over 310 kinases, we provide data transparency by supplying:
For ULight substrates:
In LANCE Ultra kinase assays, the binding of a Eu-labeled anti-phosphosubstrate antibody to the phosphorylated ULight-labeled substrate brings donor and acceptor molecules into close proximity. After irradiation of the kinase reaction at 320 or 340 nm, the energy from the Eu donor is transferred to the ULight acceptor dye, which in turn generates light at 665 nm. The intensity of the light emission is proportional to the level of ULight-substrate phosphorylation.
自動化兼容 | Yes |
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檢測方法 | Time-Resolved Fluorescence (TRF), TR-FRET |
實驗類型 | In vitro |
熒光基團 | ULight |
Format | Microplates |
分子修飾 | Phosphorylation |
產品品牌名稱 | LANCE Ultra |
運輸條件 | 幹冰 |
產品尺寸 | 0.125 nmol |
LANCE® Ultra TR-FRET reagents comprise the widest portfolio of validated kinase assay offerings available for rapid, sensitive ,and robust screening of purified kinase targets in a biochemical format.