Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. ULight™-labeled synthetic peptide containing the amino acid residues surrounding Thr389 of human p70 S6K. The ULight-p70 S6K peptide can be phosphorylated at Thr389 by several kinases including mTOR, COT, HGK, IKKε, IRAK1, IRAK4, MAP4K2, MINK, MST1, MST2, NEK1, NEK2, NEK6, NEK7, PEK, PLK1, and TAOK2. Phosphorylation motif: LGFTYVAP
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LANCE Ultra TR-FRET technology provides a two-component kinase assay that combines direct labeling of the kinase substrate with ULight™, an innovative emission dye for optimum binding efficiency, reducing steps and assay prep time. Validated for over 310 kinases, we provide data transparency by supplying:
For ULight substrates:
In LANCE Ultra kinase assays, the binding of a Eu-labeled anti-phosphosubstrate antibody to the phosphorylated ULight-labeled substrate brings donor and acceptor molecules into close proximity. After irradiation of the kinase reaction at 320 or 340 nm, the energy from the Eu donor is transferred to the ULight acceptor dye, which in turn generates light at 665 nm. The intensity of the light emission is proportional to the level of ULight-substrate phosphorylation.
| Assay Target Class | Protein |
|---|---|
| Automation Compatible | Yes |
| Detection Method | Time-Resolved Fluorescence (TRF), TR-FRET |
| Experimental Type | In vitro |
| Fluorophore | ULight |
| Format | Microplates |
| Molecular Modification | Phosphorylation |
| Product Brand Name | LANCE Ultra |
| Shipping Condition | Dry Ice |
| Therapeutic Area | Metabolic |
| Unit Size | 0.5 nmol |
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Dysregulation of kinase activity has been shown to be associated with several human diseases including cancer, diabetes and morphological disorders. Because of their function, kinases are crucial targets for drug discovery. Analysis of the human genomehas revealed the existence ofnearly 520 genes en ...
LANCE® Ultra is a high through putscreening (HTS) technology platform optimized for homogeneous time-resolved fluorescence resonance energy transfer (TRFRET) kinase assays. The LANCEUltra donor dye is a Europium(Eu) chelate, and the acceptor dye is a proprietary small-molecular weight molecule calle ...
LANCE® Ultra TR-FRET reagents comprise the widest portfolio of validated kinase assay offerings available for rapid, sensitive ,and robust screening of purified kinase targets in a biochemical format.
Protein kinases play a major role in normal cellular functions such as cell proliferation, angiogenesis, and cell adhesion. Dysregulation of kinase activity has been shown to be associated with several human diseases including cancer, diabetes and morphological disorders. This makes kinases crucial ...
Protein kinases are involved in the regulation of many aspects of the cellcycle, including proliferation, differentiation, secretion and apoptosis. Aberrant protein kinase expression or functioning is a cause orconsequence of many human diseases. As a result, kinases have become extremely popular fo ...
This LANCE Ultra kinase assay measures the phosphorylation of a p70 S6K peptide substrate at Thr389.
This LANCE Ultra kinase assay measures the phosphorylation of a p70 S6K peptide substrate at Thr389.
This LANCE Ultra kinase assay measures the phosphorylation of a p70 S6K peptide substrate at Thr389.,,
