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Western Lightning ONE Femto, Chemiluminescent Substrate (500 mL)

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A versatile, mid-level pre-mixed, one-component chemiluminescent horseradish peroxidase (HRP) substrate appropriate for a wide range of antibody dilutions, Western Lightning^™ ONE Femto can detect mid-femtogram protein levels in western blots with a high signal intensity.

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NEL140001EA

25 mL

77.00 USD

NEL141001EA

250 mL

272.00 USD

NEL142001EA

2 x 250 mL

429.00 USD

爱游戏平台注册登录 Life Sciences & Diagnostics is now Revvity, but at this time, there is no change to the supplier information.

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Overview
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Overview

Get accurate detection of both low and high abundance proteins in the same experient. Part of our premier line of premixed chemiluminescnet HRP substrates, Western Lightning ONE Femto offers the flexibility of mid-femtogram detection with excellent dynamic range. This substrate offers sensitive and consistent detection of proteins with no mixing or measuring parts required. Stored and ready-to-use at room temperature, the 12 month extended shelf life means you can buy and use Western Lightning ONE Femto with confidence. Unit size 2 x 250 mL.

Comparison of Western Lightning^™ ONE Versus Major Competitors

Performance and Signal Duration

Western blotting detection of HDAC-1 in HeLa cell lysate
Exposure time is 120 secs for each time point
Images captured with ImageQuant^™ LAS 4000 from GE Healthcare

Performance	Signal Duration
Detection Level: Mid-picogram

Two-fold dilution series of HeLa whole cell lysate from 5 μg to 0.078 μg of total protein on nitrocellulose membrane Primary antibody: Rabbit-anti Human HDAC-1 1:2000 Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) 1:20000
Detection Level: Mid-femtogram

Two-fold dilution series of HeLa whole cell lysate from 5 μg to 0.078 μg of total protein on nitrocellulose membrane Primary antibody: Rabbit-anti Human HDAC-1 1:5000 Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) 1:75000
Detection Level: Low-femtogram

Two-fold dilution series of HeLa whole cell lysate from 2.5 μg to 0.039 μg of total protein on nitrocellulose membrane Primary antibody: Rabbit-anti Human HDAC-1 1:10000 Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) 1:200000

Specifications

Detection Method	Chemiluminescence
Product Brand Name	Western Lightning ONE
Sample Type	Chemiluminescent Substrate
Shipping Condition	Ambient
Unit Size	2 x 250 mL

Resources, Events & More

Application Note

Western Blot Validation of CRISPR-Cas9-induced Functional Knockout using Synthetic sgRNA Molecules

Any well-designed CRISPR experiment must include a method to assess the accuracy and reliability of predicted gene editing results. This application note demonstrates how Horizon^™ Discovery’s Edit-R^™ predesigned synthetic sgRNAs result in high efficacy knockout o ...

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Brochure

Western Blotting Solutions

With applications in biotechnology, molecular biology, proteomics, and more, western blotting is the most widely used technique for detecting protein expression levels in cells.

Our wide range of western blotting solutions gives you the highest level of sensitivity, specificity, and select ...

PDF 2 MB

Flyer

The Power of Western Lightning ONE HRP Chemiluminescent Substrates

One-Reagent Substrates for Western Blotting

Get the consistency you demand from your western blot experiments with pre-mixed, one-reagent horseradish peroxidase (HRP) chemiluminescent substrates.

Western Lightning ONE, 爱游戏平台注册登录 ’s premier line of premixed one-component chemilumines ...

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Western Lightning Product Sheet

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