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Western Lightning ONE Femto Ultra, Chemiluminescent Substrate (25 mL)

When proteins are low and antibodies are precious, our extreme-level pre-mixed one-component chemiluminescent horseradish peroxidase (HRP) substrate with low antibody consumption is the right choice. Western Lightning ONE Femto Ultra can detect low-femtogram protein levels in western blots with superior signal intensity.

Part Number
Unit Size
List Price
Your Price
Quantity
NEL150001EA
25 mL
130.00 USD
 
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NEL151001EA
125 mL
419.00 USD
 
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NEL152001EA
2 x 125 mL
723.00 USD
 
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爱游戏平台注册登录 Life Sciences & Diagnostics is now Revvity, but at this time, there is no change to the supplier information.
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Overview

Our most sensitive substrate in our premier line of premixed chemiluminescent HRP substrates, Western Lightning ONE Femto Ultra offers low-femtogram detection with excellent signal intensity. Western Lightning ONE Femto Ultra is designed with maximum sensitivity in a single reagent format. Stored and ready-to-use at room temperature, the 12 month extended shelf life means you can buy and use Western Lightning ONE Femto Ultra with confidence. Available in 25 mL size.

Comparison of Western Lightning ONE Versus Major Competitors

Performance and Signal Duration

  • Western blotting detection of HDAC-1 in HeLa cell lysate
  • Exposure time is 120 secs for each time point
  • Images captured with ImageQuant LAS 4000 from GE Healthcare
Performance Signal Duration
Detection Level: Mid-picogram
pico pico
  • Two-fold dilution series of HeLa whole cell lysate from 5 μg to 0.078 μg of total protein on nitrocellulose membrane
  • Primary antibody: Rabbit-anti Human HDAC-1 1:2000
  • Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) 1:20000
Detection Level: Mid-femtogram
femto femto
  • Two-fold dilution series of HeLa whole cell lysate from 5 μg to 0.078 μg of total protein on nitrocellulose membrane
  • Primary antibody: Rabbit-anti Human HDAC-1 1:5000
  • Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) 1:75000
Detection Level: Low-femtogram
femto ultra femto ultra
  • Two-fold dilution series of HeLa whole cell lysate from 2.5 μg to 0.039 μg of total protein on nitrocellulose membrane
  • Primary antibody:  Rabbit-anti Human HDAC-1 1:10000
  • Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) 1:200000

Specifications

Detection Method Chemiluminescence
Product Brand Name Western Lightning ONE
Sample Type Chemiluminescent Substrate
Shipping Condition Ambient
Unit Size 25 mL
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Application Note

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Brochure

Western Blotting Solutions

With applications in biotechnology, molecular biology, proteomics, and more, western blotting is the most widely used technique for detecting protein expression levels in cells.

Our wide range of western blotting solutions gives you the highest level of sensitivity, specificity, and select ...

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Flyer

The Power of Western Lightning ONE HRP Chemiluminescent Substrates

One-Reagent Substrates for Western Blotting

Get the consistency you demand from your western blot experiments with pre-mixed, one-reagent horseradish peroxidase (HRP) chemiluminescent substrates.

Western Lightning ONE, 爱游戏平台注册登录 ’s premier line of premixed one-component chemilumines ...

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