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Western Lightning ONE Femto Ultra, Chemiluminescent Substrate (250 mL)

When proteins are low and antibodies are precious, our extreme-level pre-mixed one-component chemiluminescent horseradish peroxidase (HRP) substrate with low antibody consumption is the right choice. Western Lightning ONE Femto Ultra can detect low-femtogram protein levels in western blots with superior signal intensity.

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產品尺寸
NEL150001EA
25 mL
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NEL151001EA
125 mL
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NEL152001EA
2 x 125 mL
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詳細信息

Our most sensitive substrate in our premier line of premixed chemiluminescent HRP substrates, Western Lightning ONE Femto Ultra offers low-femtogram detection with excellent signal intensity. Western Lightning ONE Femto Ultra is designed with maximum sensitivity in a single reagent format. Stored and ready-to-use at room temperature, the 12 month extended shelf life means you can buy and use Western Lightning ONE Femto Ultra with confidence. Unit size 2 x 125 mL.

Comparison of Western Lightning ONE Versus Major Competitors

Performance and Signal Duration

  • Western blotting detection of HDAC-1 in HeLa cell lysate
  • Exposure time is 120 secs for each time point
  • Images captured with ImageQuant LAS 4000 from GE Healthcare
Performance Signal Duration
Detection Level: Mid-picogram
pico pico
  • Two-fold dilution series of HeLa whole cell lysate from 5 μg to 0.078 μg of total protein on nitrocellulose membrane
  • Primary antibody: Rabbit-anti Human HDAC-1 1:2000
  • Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) 1:20000
Detection Level: Mid-femtogram
femto femto
  • Two-fold dilution series of HeLa whole cell lysate from 5 μg to 0.078 μg of total protein on nitrocellulose membrane
  • Primary antibody: Rabbit-anti Human HDAC-1 1:5000
  • Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) 1:75000
Detection Level: Low-femtogram
femto ultra femto ultra
  • Two-fold dilution series of HeLa whole cell lysate from 2.5 μg to 0.039 μg of total protein on nitrocellulose membrane
  • Primary antibody:  Rabbit-anti Human HDAC-1 1:10000
  • Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) 1:200000

規格

檢測方法 Chemiluminescence
產品品牌名稱 Western Lightning ONE
樣品類型 Chemiluminescent Substrate
運輸條件 環境
產品尺寸 2 x 125 mL
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Western Blotting Solutions

With applications in biotechnology, molecular biology, proteomics, and more, western blotting is the most widely used technique for detecting protein expression levels in cells.

Our wide range of western blotting solutions gives you the highest level of sensitivity, specificity, and select ...

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The Power of Western Lightning ONE HRP Chemiluminescent Substrates

One-Reagent Substrates for Western Blotting

Get the consistency you demand from your western blot experiments with pre-mixed, one-reagent horseradish peroxidase (HRP) chemiluminescent substrates.

Western Lightning ONE, 爱游戏平台注册登录 ’s premier line of premixed one-component chemilumines ...

PDF 1002 KB

應用文獻

Western Blot Validation of CRISPR-Cas9-induced Functional Knockout using Synthetic sgRNA Molecules

Any well-designed CRISPR experiment must include a method to assess the accuracy and reliability of predicted gene editing results. This application note demonstrates how Horizon Discovery’s Edit-R predesigned synthetic sgRNAs result in high efficacy knockout o ...

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