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Western Lightning ONE Pico, Chemiluminescent Substrate (500 mL)

Our one-component chemiluminescent horseradish peroxidase (HRP) substrates are ideal for routine analysis detection of abundant proteins. Western Lightning™ ONE Pico can detect mid-picogram protein levels in western blots and  provides low background for a high signal to noise ratio.

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產品尺寸
NEL130001EA
25 mL
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NEL131001EA
250 mL
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NEL132001EA
2 x 250 mL
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詳細信息

Part of our premier line of premixed chemiluminescent HRP substrates, Western Lightning ONE Pico offers mid-picogram detection with no mixing required. Designed for sensitive and consistent detection of proteins, this single reagent format enables greater experimental consistency while remaining an economical choice for your everyday detection needs. Stored and ready-to-use at room temperature, the 12 month extended shelf life means you can buy and use Western Lightning ONE Pico with confidence. Unit size 2 x 250 mL.

Comparison of Western Lightning ONE Versus Major Competitors

Performance and Signal Duration

  • Western blotting detection of HDAC-1 in HeLa cell lysate
  • Exposure time is 120 secs for each time point
  • Images captured with ImageQuant LAS 4000 from GE Healthcare
Performance Signal Duration
Detection Level: Mid-picogram
pico pico
  • Two-fold dilution series of HeLa whole cell lysate from 5 μg to 0.078 μg of total protein on nitrocellulose membrane
  • Primary antibody: Rabbit-anti Human HDAC-1 1:2000
  • Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) 1:20000
Detection Level: Mid-femtogram
femto femto
  • Two-fold dilution series of HeLa whole cell lysate from 5 μg to 0.078 μg of total protein on nitrocellulose membrane
  • Primary antibody: Rabbit-anti Human HDAC-1 1:5000
  • Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) 1:75000
Detection Level: Low-femtogram
femto ultra femto ultra
  • Two-fold dilution series of HeLa whole cell lysate from 2.5 μg to 0.039 μg of total protein on nitrocellulose membrane
  • Primary antibody:  Rabbit-anti Human HDAC-1 1:10000
  • Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) 1:200000

規格

檢測方法 Chemiluminescence
產品品牌名稱 Western Lightning ONE
樣品類型 Chemiluminescent Substrate
運輸條件 環境
產品尺寸 2 x 250 mL
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Western Blotting Solutions

With applications in biotechnology, molecular biology, proteomics, and more, western blotting is the most widely used technique for detecting protein expression levels in cells.

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The Power of Western Lightning ONE HRP Chemiluminescent Substrates

One-Reagent Substrates for Western Blotting

Get the consistency you demand from your western blot experiments with pre-mixed, one-reagent horseradish peroxidase (HRP) chemiluminescent substrates.

Western Lightning ONE, 爱游戏平台注册登录 ’s premier line of premixed one-component chemilumines ...

PDF 1002 KB

應用文獻

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Any well-designed CRISPR experiment must include a method to assess the accuracy and reliability of predicted gene editing results. This application note demonstrates how Horizon Discovery’s Edit-R predesigned synthetic sgRNAs result in high efficacy knockout o ...

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