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The AlphaLISA^® SureFire^® Ultra™ total Akt1/2/3 assay is a sandwich immunoassay for quantitative detection of Akt1/2/3 (phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be a normalization or control assay in conjunction with the AlphaLISA SureFire phospho-Akt1/2/3 assay.

This kit has been pre-validated for use in combination with Pelago Bioscience’s CETSA^® (Cellular Thermal Shift Assay) technology. Please note that further assay optimization will be required in order to adapt the kit to your particular cell line and experimental conditions. A valid CETSA^® licence from Pelago Bioscience or a single kit agreement is required for using the CETSA^® technology.