PhenoVue™ Fluo-4 AM, is permeable fluorescent Ca2+ indicator and is part of Revvity’s portfolio of cellular imaging reagents. PhenoVue Fluo-4 AM Ca2+ indicator exhibits green fluorescence and is validated for use in imaging microscopy and high-content screening applications. It exhibits a maximum excitation wavelength at 495 nm and a maximum emission wavelength of 528 nm.